ELS is a light scattering technology utilized to measure the zeta potential of particles suspended in solution via Doppler shifts in scattered light. When applying an electric field on the sample, the charged particles dispersed in the suspension undergo electrophoretic movement. Due to the Doppler effect, the scattered light experiences a frequency shift compared to the incident light. The magnitude of this shift depends on particles’ electrophoretic velocity. A detector set up at the forward d
The SLS technology measures the weight-average molecular weight of macromolecules such as polymers and proteins that are dissolved in solutions. During a SLS measurements, the instrument detects scattering intensities of particles in solutions with different concentrations. The Rayleigh ratios of samples at different concentrations were calculated and plotted against concentrations into a Debye plot. The molecular weight Mw and the second virial coefficient A2 are then obtained through the inter
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